The effects of sulforaphane (4-methylsulfinylbutyl isothiocyanate), one of the most active isothiocyanates, on various tumor types have been investigated in several studies. baltica Myr-37 myrosinase Smyr37, a novel myrosinase, can be used in the preparation of isothiocyanates.Īlthough the non-isothiocyanate metabolites of glucosinolates also show some antibacterial activity, the potent anticancer activity of isothiocyanates has attracted increasing attention from researchers.
The corresponding conversion efficiency of sulforaphane from glucoraphanin was 89%. Moreover, when the reaction temperature was 40 ☌ and pH was 7.0, the crude enzyme of Smyr37 could efficiently degrade glucoraphanin into sulforaphane within 25 min with a yield of 0.57 mg/mL. The sinigrin- and glucoraphanin-hydrolyzing activities of Smyr37 were 6.95 and 5.87 U/mg, respectively. The crude enzyme of Smyr37 showed the highest activity at 50 ☌ and pH 8.0.
In this study, we described a bacterium, Shewanella baltica Myr-37, isolated from marine mud, capable of producing a novel myrosinase (Smyr37) with a molecular weight of 100 kDa. To further improve the efficiency of isothiocyanates preparation, it is necessary to explore novel sources of myrosinases. The main sources of myrosinase are cruciferous plants. Myrosinase can hydrolyze glucosinolates to generate isothiocyanates, which have cancer prevention and anti-cancer properties.
0 kommentar(er)
